Screen Printed Electrodes for Biosensor Application: Reproducibility, Sensitivity and Stability

Screen printed electrodes (SPEs) have been prepared in the Biosensor Lab @ ENEA, according to different layouts and configurations, i.e. front-back or concentric geometry. Different pastes and composition have been tested depending on the application and requirements for flow or batch measurements. In order to optimize the quality of the electrochemical response, graphite pastes and metal doped inks have been used, varying their physical features and composition. Preparation and analytical evaluation of SPEs probes have shown useful for specific detection of contaminants in complex matrices or for online monitoring their toxicological effect. Electrochemical comparison between commercially available probes and home-produced SPEs and statistical evaluation have been performed, to verify reproducibility at medium scale. Higher sensitivity and stability of these new probes have been achieved both in amperometric flow conditions and in voltammetric measurements. The results obtained show the possibility to use our SPEs, especially those based on Gwent Electronic Materials pastes for several reliable sensing applications.